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Research Paper Abstract Introduction

Psych./Neuro. 201

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An APA-style paper includes the following sections: title page, abstract, introduction, method, results, discussion, and references. Your paper may also include one or more tables and/or figures. Different types of information about your study are addressed in each of the sections, as described below. 

 

General formatting rules are as follows:

  • Do not put page breaks in between the introduction, method, results, and discussion sections.
  • The title page, abstract, references, table(s), and figure(s) should be on their own pages.
  • The entire paper should be written in the past tense, in a 12-point font, double-spaced, and with one-inch margins all around.


Title page 

(see sample on p. 41 of APA manual)

  • Title should be between 10-12 words and should reflect content of paper (e.g., IV and DV).
  • Title, your name, and Hamilton College are all double-spaced (no extra spaces)
  • Create a page header using the “View header” function in MS Word. On the title page, the header should include the following:
    • Flush left: Running head: THE RUNNING HEAD SHOULD BE IN ALL CAPITAL LETTERS. The running head is a short title that appears at the top of pages of published articles. It should not exceed 50 characters, including punctuation and spacing. (Note: on the title page, you actually write the words Running head, but these words do not appear on subsequent pages; just the actual running head does. If you make a section break between the title page and the rest of the paper you can make the header different for those two parts of the manuscript).
    • Flush right, on same line: page number. Use the toolbox to insert a page number, so it will automatically number each page.

Abstract 

(labeled, centered, not bold)

  • No more than 120 words, one paragraph, block format (i.e., don’t indent), double-spaced.
  • State topic, preferably in one sentence. Provide overview of method, results, and discussion

Introduction 

(Do not label as “Introduction.” Title of paper goes at the top of the page—not bold)

The introduction of an APA-style paper is the most difficult to write. A good introduction will summarize, integrate, and critically evaluate the empirical knowledge in the relevant area(s) in a way that sets the stage for your study and why you conducted it. The introduction starts out broad (but not too broad!) and gets more focused toward the end. Here are some guidelines for constructing a good introduction:

  • Don’t put your readers to sleep by beginning your paper with the time-worn sentence, Past research has shown....(blah blah blah) They’ll be snoring within a paragraph! Try to draw your reader in by saying something interesting or thought-provoking right off the bat. Take a look at articles you’ve read. Which ones captured your attention right away? How did the authors accomplish this task? Which ones didn’t? Why not? See if you can use articles you liked as a model. One way to begin (but not the only way) is to provide an example or anecdote illustrative of your topic area.
  • Although you won’t go into the details of your study and hypotheses until the end of the intro, you should foreshadow your study a bit at the end of the first paragraph by stating your purpose briefly, to give your reader a schema for all the information you will present next.
  • Your intro should be a logical flow of ideas that leads up to your hypothesis. Try to organize it in terms of the ideas rather than who did what when. In other words, your intro shouldn’t read like a story of “Schmirdley did such-and-such in 1991. Then Gurglehoff did something-or-other in 1993. Then....(etc.)” First, brainstorm all of the ideas you think are necessary to include in your paper. Next, decide which ideas make sense to present first, second, third, and so forth, and think about how you want to transition between ideas. When an idea is complex, don’t be afraid to use a real-life example to clarify it for your reader. The introduction will end with a brief overview of your study and, finally, your specific hypotheses. The hypotheses should flow logically out of everything that’s been presented, so that the reader has the sense of, “Of course. This hypothesis makes complete sense, given all the other research that was presented.”
  • When incorporating references into your intro, you do not necessarily need to describe every single study in complete detail, particularly if different studies use similar methodologies. Certainly you want to summarize briefly key articles, though, and point out differences in methods or findings of relevant studies when necessary. Don’t make one mistake typical of a novice APA-paper writer by stating overtly why you’re including a particular article (e.g., “This article is relevant to my study because…”). It should be obvious to the reader why you’re including a reference without your explicitly saying so. DO NOT quote from the articles, instead paraphrase by putting the information in your own words.
  • Be careful about citing your sources (see APA manual). Make sure there is a one-to-one correspondence between the articles you’ve cited in your intro and the articles listed in your reference section.
  • Remember that your audience is the broader scientific community, not the other students in your class or your professor. Therefore, you should assume they have a basic understanding of psychology, but you need to provide them with the complete information necessary for them to understand the research you are presenting.

Method 

(labeled, centered,  bold)

The Method section of an APA-style paper is the most straightforward to write, but requires precision. Your goal is to describe the details of your study in such a way that another researcher could duplicate your methods exactly. The Method section typically includes Participants, Materials and/or Apparatus, and Procedure sections. If the design is particularly complicated (multiple IVs in a factorial experiment, for example), you might also include a separate Design subsection or have a “Design and Procedure” section. Note that in some studies (e.g., questionnaire studies in which there are many measures to describe but the procedure is brief), it may be more useful to present the Procedure section prior to the
Materials section rather than after it.

Participants 

(labeled, flush left, bold)

  • Total number of participants (# women, # men), age range, mean and SD for age, racial/ethnic composition (if applicable), population type (e.g., college students). Remember to write numbers out when they begin a sentence.
  • How were the participants recruited? (Don’t say “randomly” if it wasn’t random!) Were they compensated for their time in any way? (e.g., money, extra credit points)
  • Write for a broad audience. Thus, do not write, “Students in Psych. 280...” Rather, write (for instance), “Students in a psychological statistics and research methods course at a small liberal arts college….”
  • Try to avoid short, choppy sentences. Combine information into a longer sentence when possible.

Materials 

(labeled, flush left, bold)

Carefully describe any stimuli, questionnaires, and so forth. It is unnecessary to mention things such as the paper and pencil used to record the responses, the data recording sheet, the computer that ran the data analysis, the color of the computer, and so forth. If you included a questionnaire, you should describe it in detail. For instance, note how many items were on the questionnaire, what the response format was (e.g., a 5-point Likert-type scale ranging from 1 (strongly disagree) to 5 (strongly agree)), how many items were reverse-scored, whether the measure had subscales, and so forth. Provide a sample item or two for your reader. If you have created a new instrument, you should attach it as an Appendix. If you presented participants with various word lists to remember or stimuli to judge, you should describe those in detail here. Use subheadings to separate different types of stimuli if needed. If you are only describing questionnaires, you may call this section “Measures.”

Apparatus

(labeled, flush left, bold)

Include an apparatus section if you used specialized equipment for your study (e.g., the eyetracking machine) and need to describe it in detail.

Procedure

(labeled, flush left, bold)

What did participants do, and in what order? When you list a control variable (e.g., “Participants all sat two feet from the experimenter.”), explain WHY you did what you did. In other words, what nuisance variable were you controlling for? Your procedure should be as brief and concise as possible. Read through it. Did you repeat yourself anywhere? If so, how can you rearrange things to avoid redundancy? You may either write the instructions to the participants verbatim or paraphrase, whichever you deem more appropriate. Don’t forget to include brief statements about informed consent and debriefing.

Results

(labeled, centered, bold)

In this section, describe how you analyzed the data and what you found. If your data analyses were complex, feel free to break this section down into labeled subsections, perhaps one section for each hypothesis.

  • Include a section for descriptive statistics
  • List what type of analysis or test you conducted to test each hypothesis.
  • Refer to your Statistics textbook for the proper way to report results in APA style. A t-test, for example, is reported in the following format: t (18) = 3.57, p < .001, where 18 is the number of degrees of freedom (N – 2 for an independentgroups t test). For a correlation: r (32) = -.52, p < .001, where 32 is the number of degrees of freedom (N – 2 for a correlation). For a one-way ANOVA: F (2, 18) = 7.00, p < .001, where 2 represents the dfbetween and 18 represents dfwithin. Remember that if a finding has a p value greater than .05, it is “nonsignificant,”  not “insignificant.” For nonsignificant findings, still provide the exact p values. For correlations, be sure to report the rvalue as an assessment of the strength of the finding, to show what proportion of variability is shared by the two variables you’re correlating. For t- tests and ANOVAs, report eta2.
  • Report exact p values to two or three decimal places (e.g., p = .042; see p. 114 of APA manual). However, for pvalues less than .001, simply put p < .001.
  • Following the presentation of all the statistics and numbers, be sure to state the nature of your finding(s) in words and whether or not they support your hypothesis (e.g., “As predicted, …”). This information can typically be presented in a sentence or two following the numbers (within the same paragraph). Also, be sure to include the relevant means and SDs.
  • It may be useful to include a table or figure to represent your results visually. Be sure to refer to these in your paper (e.g., “As illustrated in Figure 1…”). Remember that you may present a set of findings either as a table or as a figure, but not as both. Make sure that your text is not redundant with your tables/figures. For instance, if you present a table of means and standard deviations, you do not need to also report these in the text. However, if you use a figure to represent your results, you may wish to report means and standard deviations in the text, as these may not always be precisely ascertained by examining the figure. Do describe the trends shown in the figure.
  • Do not spend any time interpreting or explaining the results; save that for the Discussion section.

Discussion 

(labeled, centered, bold)

The goal of the discussion section is to interpret your findings and place them in the broader context of the literature in the area. A discussion section is like the reverse of the introduction, in that you begin with the specifics and work toward the more general (funnel out). Some points to consider:

  • Begin with a brief restatement of your main findings (using words, not numbers). Did they support the hypothesis or not? If not, why not, do you think? Were there any surprising or interesting findings?
  • How do your findings tie into the existing literature on the topic, or extend previous research? What do the results say about the broader behavior under investigation? Bring back some of the literature you discussed in the Introduction, and show how your results fit in (or don’t fit in, as the case may be). If you have surprising findings, you might discuss other theories that can help to explain the findings. Begin with the assumption that your results are valid, and explain why they might differ from others in the literature.
  • What are the limitations of the study? If your findings differ from those of other researchers, or if you did not get statistically significant results, don’t spend pages and pages detailing what might have gone wrong with your study, but do provide one or two suggestions. Perhaps these could be incorporated into the future research section, below.
  • What additional questions were generated from this study? What further research should be conducted on the topic? What gaps are there in the current body of research? Whenever you present an idea for a future research study, be sure to explain why you think that particular study should be conducted. What new knowledge would be gained from it? Don’t just say, “I think it would be interesting to re-run the study on a different college campus" or "It would be better to run the study again with more participants.” Really put some thought into what extensions of the research might be interesting/informative, and why.
  • What are the theoretical and/or practical implications of your findings? How do these results relate to larger issues of human thoughts, feelings, and behavior? Give your readers “the big picture.” Try to answer the question, “So what?”
  • Final paragraph: Be sure to sum up your paper with a final concluding statement. Don’t just trail off with an idea for a future study. End on a positive note by reminding your reader why your study was important and what it added to the literature.

References 

(labeled, centered, not bold)

Provide an alphabetical listing of the references (alphabetize by last name of first author). Double-space all, with no extra spaces between references. The second line of each reference should be indented (this is called a hanging indent and is easily accomplished using the ruler in Microsoft Word). See the APA manual for how to format references correctly. Examples of references to journal articles start on p. 198 of the manual, and examples of references to books and book chapters start on pp. 202. Digital object identifiers (DOIs) are now included for electronic sources (see pp. 187-192 of APA manual to learn more).

Journal article example:

[Note that only the first letter of the first word of the article title is capitalized; the journal name and volume are italicized. If the journal name had multiple words, each of the major words would be capitalized.]

Ebner-Priemer, U. W., & Trull, T. J. (2009). Ecological momentary assessment of mood disorders and mood dysregulation. Psychological Assessment, 21, 463-475. doi:10.1037/a0017075


Book chapter example:

[Note that only the first letter of the first word of both the chapter title and book title are capitalized.]

Stephan, W. G. (1985). Intergroup relations. In G. Lindzey & E. Aronson (Eds.), The handbook of social psychology (3rd ed., Vol. 2, pp. 599-658). New York: Random House.


Book example:

Gray, P. (2010). Psychology (6th ed.). New York: Worth

Table

There are various formats for tables, depending upon the information you wish to include. See the APA manual. Be sure to provide a table number and table title (the latter is italicized). Tables can be single or double-spaced.

Figure

If you have more than one figure, each one gets its own page. Use a sans serif font, such as Helvetica, for any text within your figure. Be sure to label your x- and y-axes clearly, and make sure you’ve noted the units of measurement of the DV. Underneath the figure provide a label and brief caption (e.g., ―Figure 1. Mean evaluation of job applicant qualifications as
a function of applicant attractiveness level‖). The figure caption typically includes the IVs/predictor variables and the DV. Include error bars in your bar graphs, and note what the bars represent in the figure caption: Error bars represent one standard error above and below the mean.

In-Text Citations

(see pp. 174-179 of APA manual)

When citing sources in your paper, you need to include the authors’ names and publication date. You should use the following formats:

When including the citation as part of the sentence, use AND: “According to Jones and Smith (2003), the…”

When the citation appears in parentheses, use “&”: “Studies have shown that priming can affect actual motor behavior (Jones & Smith, 2003; Klein, Bailey, & Hammer, 1999).” The studies appearing in parentheses should be ordered alphabetically by the first author’s last name, and should be separated by semicolons.

If you are quoting directly (which you should avoid), you also need to include the page number.

For sources with three or more authors, once you have listed all the authors’ names, you may write “et al.” on subsequent mentions. For example: “Klein et al. (1999) found that....” For sources with two authors, both authors must be included every time the source is cited. When a source has six or more authors, the first author’s last name and “et al.” are used every time the source is cited (including the first time).

Secondary Sources

“Secondary source” is the term used to describe material that is cited in another source. If in his article entitled “Behavioral Study of Obedience” (1963), Stanley Milgram makes reference to the ideas of Snow (presented above), Snow (1961) is the primary source, and Milgram (1963) is the secondary source. Try to avoid using secondary sources in your
papers; in other words, try to find the primary source and read it before citing it in your own work. If you must use a secondary source, however, you should cite it in the following way:

Snow (as cited in Milgram, 1963) argued that, historically, the cause of most criminal acts... 

The reference for the Milgram article (but not the Snow reference) should then appear in the reference list at the end of your paper.

Writing Research Papers

Writing is easy. All you do is stare at a blank sheet of paper until drops of blood form on your forehead. --- Gene Fowler

A major goal of this course is the development of effective technical writing skills. To help you become an accomplished writer, you will prepare several research papers based upon the studies completed in lab. Our research papers are not typical "lab reports." In a teaching lab a lab report might be nothing more than answers to a set of questions. Such an assignment hardly represents the kind of writing you might be doing in your eventual career.

Written and oral communications skills are probably the most universal qualities sought by graduate and professional schools as well as by employers. You alone are responsible for developing such skills to a high level.

Resources for learning technical writing

Before you begin your first writing assignment, please consult all of the following resources, in order to gain the most benefit from the experience.

  • General form of a typical research article
  • Specific guidelines (if any) for the assignment – see the writeups on individual lab studies
  • McMillan, VE. "Writing Papers in the Biological Sciences, Third Ed." New York: Bedford/St. Martin's, 2001. ISBN 0-312-25857-7 (REQUIRED for Bioc 211, 311, recommended for other science courses that include writing)
  • Writing portfolio examples (pdf)

As you polish up your writing skills please make use of the following resources

For Biosciences majors the general guidelines apply to future course work, as can be seen by examining the guidelines for the advanced experimental sciences research paper (Bioc 311).

General form of a research paper

An objective of organizing a research paper is to allow people to read your work selectively. When I research a topic, I may be interested in just the methods, a specific result, the interpretation, or perhaps I just want to see a summary of the paper to determine if it is relevant to my study. To this end, many journals require the following sections, submitted in the order listed, each section to start on a new page. There are variations of course. Some journals call for a combined results and discussion, for example, or include materials and methods after the body of the paper. The well known journal Science does away with separate sections altogether, except for the abstract.

Your papers are to adhere to the form and style required for the Journal of Biological Chemistry, requirements that are shared by many journals in the life sciences.

General style

Specific editorial requirements for submission of a manuscript will always supercede instructions in these general guidelines.

To make a paper readable

  • Print or type using a 12 point standard font, such as Times, Geneva, Bookman, Helvetica, etc.
  • Text should be double spaced on 8 1/2" x 11" paper with 1 inch margins, single sided
  • Number pages consecutively
  • Start each new section on a new page
  • Adhere to recommended page limits

Mistakes to avoid

  • Placing a heading at the bottom of a page with the following text on the next page (insert a page break!)
  • Dividing a table or figure - confine each figure/table to a single page
  • Submitting a paper with pages out of order

In all sections of your paper

  • Use normal prose including articles ("a", "the," etc.)
  • Stay focused on the research topic of the paper
  • Use paragraphs to separate each important point (except for the abstract)
  • Indent the first line of each paragraph
  • Present your points in logical order
  • Use present tense to report well accepted facts - for example, 'the grass is green'
  • Use past tense to describe specific results - for example, 'When weed killer was applied, the grass was brown'
  • Avoid informal wording, don't address the reader directly, and don't use jargon, slang terms, or superlatives
  • Avoid use of superfluous pictures - include only those figures necessary to presenting results

Title Page

Select an informative title as illustrated in the examples in your writing portfolio example package. Include the name(s) and address(es) of all authors, and date submitted. "Biology lab #1" would not be an informative title, for example.

Abstract

The summary should be two hundred words or less. See the examples in the writing portfolio package.

General intent

An abstract is a concise single paragraph summary of completed work or work in progress. In a minute or less a reader can learn the rationale behind the study, general approach to the problem, pertinent results, and important conclusions or new questions.

Writing an abstract

Write your summary after the rest of the paper is completed. After all, how can you summarize something that is not yet written? Economy of words is important throughout any paper, but especially in an abstract. However, use complete sentences and do not sacrifice readability for brevity. You can keep it concise by wording sentences so that they serve more than one purpose. For example, "In order to learn the role of protein synthesis in early development of the sea urchin, newly fertilized embryos were pulse-labeled with tritiated leucine, to provide a time course of changes in synthetic rate, as measured by total counts per minute (cpm)." This sentence provides the overall question, methods, and type of analysis, all in one sentence. The writer can now go directly to summarizing the results.

Summarize the study, including the following elements in any abstract. Try to keep the first two items to no more than one sentence each.

  • Purpose of the study - hypothesis, overall question, objective
  • Model organism or system and brief description of the experiment
  • Results, including specific data - if the results are quantitative in nature, report quantitative data; results of any statistical analysis shoud be reported
  • Important conclusions or questions that follow from the experiment(s)

Style:

  • Single paragraph, and concise
  • As a summary of work done, it is always written in past tense
  • An abstract should stand on its own, and not refer to any other part of the paper such as a figure or table
  • Focus on summarizing results - limit background information to a sentence or two, if absolutely necessary
  • What you report in an abstract must be consistent with what you reported in the paper
  • Corrrect spelling, clarity of sentences and phrases, and proper reporting of quantities (proper units, significant figures) are just as important in an abstract as they are anywhere else

Introduction

Your introductions should not exceed two pages (double spaced, typed). See the examples in the writing portfolio package.

General intent

The purpose of an introduction is to aquaint the reader with the rationale behind the work, with the intention of defending it. It places your work in a theoretical context, and enables the reader to understand and appreciate your objectives.

Writing an introduction

The abstract is the only text in a research paper to be written without using paragraphs in order to separate major points. Approaches vary widely, however for our studies the following approach can produce an effective introduction.

  • Describe the importance (significance) of the study - why was this worth doing in the first place? Provide a broad context.
  • Defend the model - why did you use this particular organism or system? What are its advantages? You might comment on its suitability from a theoretical point of view as well as indicate practical reasons for using it.
  • Provide a rationale. State your specific hypothesis(es) or objective(s), and describe the reasoning that led you to select them.
  • Very briefy describe the experimental design and how it accomplished the stated objectives.

Style:

  • Use past tense except when referring to established facts. After all, the paper will be submitted after all of the work is completed.
  • Organize your ideas, making one major point with each paragraph. If you make the four points listed above, you will need a minimum of four paragraphs.
  • Present background information only as needed in order support a position. The reader does not want to read everything you know about a subject.
  • State the hypothesis/objective precisely - do not oversimplify.
  • As always, pay attention to spelling, clarity and appropriateness of sentences and phrases.

Materials and Methods

There is no specific page limit, but a key concept is to keep this section as concise as you possibly can. People will want to read this material selectively. The reader may only be interested in one formula or part of a procedure. Materials and methods may be reported under separate subheadings within this section or can be incorporated together.

General intent

This should be the easiest section to write, but many students misunderstand the purpose. The objective is to document all specialized materials and general procedures, so that another individual may use some or all of the methods in another study or judge the scientific merit of your work. It is not to be a step by step description of everything you did, nor is a methods section a set of instructions. In particular, it is not supposed to tell a story. By the way, your notebook should contain all of the information that you need for this section.

Writing a materials and methods section

Materials:

  • Describe materials separately only if the study is so complicated that it saves space this way.
  • Include specialized chemicals, biological materials, and any equipment or supplies that are not commonly found in laboratories.
  • Do not include commonly found supplies such as test tubes, pipet tips, beakers, etc., or standard lab equipment such as centrifuges, spectrophotometers, pipettors, etc.
  • If use of a specific type of equipment, a specific enzyme, or a culture from a particular supplier is critical to the success of the experiment, then it and the source should be singled out, otherwise no.
  • Materials may be reported in a separate paragraph or else they may be identified along with your procedures.
  • In biosciences we frequently work with solutions - refer to them by name and describe completely, including concentrations of all reagents, and pH of aqueous solutions, solvent if non-aqueous.
Methods:
  • See the examples in the writing portfolio package
  • Report the methodology (not details of each procedure that employed the same methodology)
  • Describe the mehodology completely, including such specifics as temperatures, incubation times, etc.
  • To be concise, present methods under headings devoted to specific procedures or groups of procedures
  • Generalize - report how procedures were done, not how they were specifically performed on a particular day. For example, report "samples were diluted to a final concentration of 2 mg/ml protein;" don't report that "135 microliters of sample one was diluted with 330 microliters of buffer to make the protein concentration 2 mg/ml." Always think about what would be relevant to an investigator at another institution, working on his/her own project.
  • If well documented procedures were used, report the procedure by name, perhaps with reference, and that's all. For example, the Bradford assay is well known. You need not report the procedure in full - just that you used a Bradford assay to estimate protein concentration, and identify what you used as a standard. The same is true for the SDS-PAGE method, and many other well known procedures in biology and biochemistry.
Style:
  • It is awkward or impossible to use active voice when documenting methods without using first person, which would focus the reader's attention on the investigator rather than the work. Therefore when writing up the methods most authors use third person passive voice.
  • Use normal prose in this and in every other section of the paper – avoid informal lists, and use complete sentences.

What to avoid

  • Materials and methods are not a set of instructions.
  • Omit all explanatory information and background - save it for the discussion.
  • Omit information that is irrelevant to a third party, such as what color ice bucket you used, or which individual logged in the data.

Results

The page length of this section is set by the amount and types of data to be reported. Continue to be concise, using figures and tables, if appropriate, to present results most effectively. See recommendations for content, below.

General intent

The purpose of a results section is to present and illustrate your findings. Make this section a completely objective report of the results, and save all interpretation for the discussion.

Writing a results section

IMPORTANT: You must clearly distinguish material that would normally be included in a research article from any raw data or other appendix material that would not be published. In fact, such material should not be submitted at all unless requested by the instructor.

Content

  • Summarize your findings in text and illustrate them, if appropriate, with figures and tables.
  • In text, describe each of your results, pointing the reader to observations that are most relevant.
  • Provide a context, such as by describing the question that was addressed by making a particular observation.
  • Describe results of control experiments and include observations that are not presented in a formal figure or table, if appropriate.
  • Analyze your data, then prepare the analyzed (converted) data in the form of a figure (graph), table, or in text form.

What to avoid

  • Do not discuss or interpret your results, report background information, or attempt to explain anything.
  • Never include raw data or intermediate calculations in a research paper.
  • Do not present the same data more than once.
  • Text should complement any figures or tables, not repeat the same information.
  • Please do not confuse figures with tables - there is a difference.

Style

  • As always, use past tense when you refer to your results, and put everything in a logical order.
  • In text, refer to each figure as "figure 1," "figure 2," etc. ; number your tables as well (see the reference text for details)
  • Place figures and tables, properly numbered, in order at the end of the report (clearly distinguish them from any other material such as raw data, standard curves, etc.)
  • If you prefer, you may place your figures and tables appropriately within the text of your results section.

Figures and tables

  • Either place figures and tables within the text of the result, or include them in the back of the report (following Literature Cited) - do one or the other
  • If you place figures and tables at the end of the report, make sure they are clearly distinguished from any attached appendix materials, such as raw data
  • Regardless of placement, each figure must be numbered consecutively and complete with caption (caption goes under the figure)
  • Regardless of placement, each table must be titled, numbered consecutively and complete with heading (title with description goes above the table)
  • Each figure and table must be sufficiently complete that it could stand on its own, separate from text

Discussion

Journal guidelines vary. Space is so valuable in the Journal of Biological Chemistry, that authors are asked to restrict discussions to four pages or less, double spaced, typed. That works out to one printed page. While you are learning to write effectively, the limit will be extended to five typed pages. If you practice economy of words, that should be plenty of space within which to say all that you need to say.

General intent

The objective here is to provide an interpretation of your results and support for all of your conclusions, using evidence from your experiment and generally accepted knowledge, if appropriate. The significance of findings should be clearly described.

Writing a discussion

Interpret your data in the discussion in appropriate depth. This means that when you explain a phenomenon you must describe mechanisms that may account for the observation. If your results differ from your expectations, explain why that may have happened. If your results agree, then describe the theory that the evidence supported. It is never appropriate to simply state that the data agreed with expectations, and let it drop at that.

  • Decide if each hypothesis is supported, rejected, or if you cannot make a decision with confidence. Do not simply dismiss a study or part of a study as "inconclusive."
  • Research papers are not accepted if the work is incomplete. Draw what conclusions you can based upon the results that you have, and treat the study as a finished work
  • You may suggest future directions, such as how the experiment might be modified to accomplish another objective.
  • Explain all of your observations as much as possible, focusing on mechanisms.
  • Decide if the experimental design adequately addressed the hypothesis, and whether or not it was properly controlled.
  • Try to offer alternative explanations if reasonable alternatives exist.
  • One experiment will not answer an overall question, so keeping the big picture in mind, where do you go next? The best studies open up new avenues of research. What questions remain?
  • Recommendations for specific papers will provide additional suggestions.
Style:
  • When you refer to information, distinguish data generated by your own studies from published information or from information obtained from other students (verb tense is an important tool for accomplishing that purpose).
  • Refer to work done by specific individuals (including yourself) in past tense.
  • Refer to generally accepted facts and principles in present tense. For example, "Doofus, in a 1989 survey, found that anemia in basset hounds was correlated with advanced age. Anemia is a condition in which there is insufficient hemoglobin in the blood."

The biggest mistake that students make in discussions is to present a superficial interpretation that more or less re-states the results. It is necessary to suggest why results came out as they did, focusing on the mechanisms behind the observations.

Literature Cited

Please note that in the introductory laboratory course, you will not be required to properly document sources of all of your information. One reason is that your major source of information is this website, and websites are inappropriate as primary sources. Second, it is problematic to provide a hundred students with equal access to potential reference materials. You may nevertheless find outside sources, and you should cite any articles that the instructor provides or that you find for yourself.

List all literature cited in your paper, in alphabetical order, by first author. In a proper research paper, only primary literature is used (original research articles authored by the original investigators). Be cautious about using web sites as references - anyone can put just about anything on a web site, and you have no sure way of knowing if it is truth or fiction. If you are citing an on line journal, use the journal citation (name, volume, year, page numbers). Some of your papers may not require references, and if that is the case simply state that "no references were consulted."

Vietnamese translation: http://translate.coupofy.com/writing-research-papers/
Russian translation: http://blog.hightwall.com/reportform/